Extraction and separation of Antioxidative Compounds from Salvia leriifolia Leaves

In this research, methanolic extraction of Salvia leriifolia leaves produced a higher yield and antioxidative activity than other organic solvents (ethanol, acetone, chloroform, n-hexane). ‏The methanolic extract was reserved about 24 hours in the refrigerator and its precipitates were then separated. The extract was separated into 12 fractions by thin-layer chromatography (TLC). The highest yields were found in fractions with Rf values of 0.29, 0.54, 0.11 and 0.38 at 16.24%, 12.48%, 8.81% and 7.60%, respectively. All fractions and also whole methanolic extract and precipitates of methanolic extract had more antioxidative activity than the control based on the thiocyanate method. Whole methanolic extract, precipitates of methanolic extract and most separated fractions showed more antioxidative activity than a–tocopherol. The fraction with Rf value of 0.29 at 16.24% yield and 85.61% antioxidative activity of synthetic antioxidant BHT based on thiocyanat method was chosen as the fraction with the highest yield and the highest antioxidative activity.