Reaction of Wheat Varieties to Infection by Pythium arrhenomanes or its Toxic Metabolite (s)

Eight wheat varieties some of which were earlier reported to be resistant to arrhennmanes, placing 2-day-old seedlings on the edge of a Pythiumwere inoculated with P. arrhenomanes. Inoculation was performed byP. arrhenomanes culture on corn mealagar dia.) containing glass beads and 1 ml of sterile water, and incubated for 4 days at25°O Non-inoculated seedlings were exposed to the toxic metabolite(s) from arrhenomanes filtrate of sterile water served as controls. Measurements were taken on root and shoot length,as well as fresh and dry root and shoot weights. There was an indication that it maybe possible to use the toxic metabolite(s) in screening for resistance to arrhenomanes. some differences among them in their reaction to fungal infection or exposure to thetoxic metabolite(s).for 3 h at 25°C. Infected seedlings were then transferred to test tubes (9 mmP.by placing them in test tubes containing 1 ml of a 25-day-old cultureP. arrhenomanes. Non-inoculated seedlings grown in test tubes containingP.Although all of the varieties appeared to be susceptible, there were