Responses of Selected Plants to Petroleum Contamination in Soils Around the Tehran Oil Refinery

Total petroleum hydrocarbon (TPH) contaminations in soils may be toxic to human, plants and cause groundwater contamination. To achieve maximum TpH- reduction and to establish successfull stable vegetation cover in phytoremediation method, various criteria must be considered to choose the plants carefully. In this study, germination and subsequent growth of seven plants were tested in three soils with different petroleum contamination levels. Contamination treatments consisted of C0 (uncontaminated soil), C1 (1:1 w/w, uncontaminated: contaminated soil) and C2 (1:3 w/w, uncontaminated: contaminated soil). The experimental design was completely randomized split plots with three replications per treatment. The results showed that the presence of TPH in the soil had no effect on seed germination of agropyron, white clover, sunflower and safflower although canola seedlings were sensitive to these compounds and failed to produce dry matter yield (DMY) at the end of trial period. In contrast, seed germination of canola, puccenillia and tall fescue decreased in the petroleum contaminated soils. No reduction was found in DMY of puccenillia in contaminated soils (C1 and C2 treatments) compared to control however, the presence of TPH proportional to the contamination levels, decreased dry weight of sunflower and safflower. This reduction in growth and dry weight for tall fescue and agropyron was also observed in C2 compared to C1 treatment. Therefore, it seems that though agropyron, white clover, sunflower and safflower germinated well and the presence of TPHs in the soil treatments had no effect on their seed germination, they grew poorly. In contrast, grasses had poor seed germination but their subsequent growth and establishment in the contaminated soils was acceptable for subsequent phytoremediation trials.