A Comparison of Culture and PCR Methods Wrights Test for Diagnosis of Brucellosis

Brucellosis is still known as a main hygienic problem in human. Pathogen is easily transmitted into human body by raw milk and fresh cheese. The use of rapid and sensitive diagnostic methods is essential because of the hygienic and economic importance of brucellosis. In this research, we collected 106 samples of milk and blood serum from dairy cattle that had been referred to Rey slaughterhouse. Milk samples were cultured in modified Farrell ' s medium. In addition, according to Romero method, DNA was extracted from milk samples and PCR was done using JPR and JPF primers. Wright test was also done on serum samples. Comparison of PCR and culture with Wright test results showed that in culture method sensitivity is 31.6 percent and accuracy is 48 percent but in PCR, sensitivity is 58 percent and accuracy is 65 percent. Results of our study showed that PCR can be attended as a suitable and rapid method to diagnose brucellosis.