Comparison of Haemagglutination Inhibition and Single Radial Immunodiffusion Tests for the Detection of Influenza Virus Antibodies

Influenza is one of the most contagious dieases in human and other animals. Previous infection with the influenza virus can be shown by showing specific antibodies to the virus. Using single radial diffusion est (SRD), haemagglutination inhibition test (HII), and viral neutralization test (VNT), we tried to detect nd measure influenza virus type-B antibodies.In order to run the above tests viral antigens were first prepared in chick embyonated eggs and cell culture separately. Specific antibodies were prepared in healthy rabbits injected with the above antigens.The SRD, HII, and VNT were done using the above materials. Serum samples were tested for influenza ypeB virus antibodies.The VNT was taken as the standard test and the results obtaind from the other tests were compared with those of the VNT, The sensitivity and specificity of HIT and SRD were 93% and 84%, 85% and 89% respectively.