Seasonal alteration of peroxidase in branch and leaves of Eucalyptus viminalis Labill.

The morphology of deciduous plants changes seasonally. In addition, deciduous and ever-green plants encounter with internal changes as a consequence of seasons. Enzymes activities alter with changes of temperature, drought and salinity. Plants adapt to environmental changes with alteration of enzymes activities. In this study, the peroxidase activity was measured in leaves and shoots of Eucalyptus viminalis during a year. Peroxidase activity was measured by spectrophotometer (530 nm) by use of Worthington method. Data were analyzed to determine statistical relationship in peroxidase activity of individual trees and seasonal changes of enzyme activity by SPSS software. Results showed that peroxidase activity was different in individual trees. The difference of peroxidase activity in different individual trees was little in April, July and January but differences were higher in September and November. The peroxidase activity decreased in all individual trees from April to July. Peroxidase activity of leaves was higher than those of branches in all of studied months. There was negative correlation between some month of peroxidase activity (α<0.05). There was not any correlation between leaves and branches peroxidase activity. Individuals were classified to three classes according to their peroxidase activities using JMP software (centroid method).