Cervicovaginal Cytopathology by Liquiprep"! a New Liquid BasedMethod in Comparison with Conventional Pap Smear

The aim of this study was to compare the screening performance ofa new modified liquid-based cytology method (Liquiprep™) with conventional PAP smear (CP) ina low risk population, using colposcopy followed by histology as “gold standard”.Patients and This cross-sectional study was performed on random referred specimensto a general gynecological clinic in Tehran, during 20 months by a split-sample method. In bothCP and Liquiprep™ group, all positive and 10% of negative results of smears were followed bycolposcopy. A biopsy was taken whenever any atypical transformation zone seen. Sensitivity,specificity, negative and positive predictive values (PV), and overall accuracy of both methods wereanalyzed in relation to histology. A total of 1265 patients were analyzed by two methods. In 158 (12.5%) of cases histologicaldiagnosis was made. Liquiprep™ samples (94.7%) were more adequate than CP (92.1%). Therewas not any low or high-grade squamous intraepithelial lesion (SIL). Atypical squamous cell ofundetermined significance (ASC-US) was diagnosed more with CP than with Liquiprep™ (1.43%vs. 0.79%) while pathologically 60% of ASC-US in Liquiprep™ and 16.6% in CP had degrees ofSIL. The Liquiprep™ had a significantly higher sensitivity (83% vs. 66%) and positive PV (83%vs. 33%) than the CP to detect SIL at histology but the difference in specificity was non significant(98% vs. 86%). This study confirms the superiority of the Liquiprep™ method to detect cervicallesions in a low risk population.