Effects of plant growth regulators NAA, BAP, different explants scale and photoperiod on tissue culture of Lilium ledebourii Boiss

Lilium ledebourii Boiss is a native flower in the north of Iran, however it is an endangered species. In order to introduce and domesticate endemic plants, the first step is evaluation for propagation. One of the best and most propogation vegetative method of Lilies is in vitro scale culture using plant growth regulators in different condition. This experiment conducted using a completly randomized design (CRC) with four factors. 1- Light period at two levels:A:Lightness (16 hr light and 8 hr dark) B:darkness (24 hr dark). 2- Regional explant at two levels: A:Half distal scale, B:Half basal scale. 3- Concentrations of 6-benzylamynopurine (BAP) at three levels A:Zero or control B:0.01mg1-1 C:0.1mg1-1. 4- Concentrations of -naphtalen acetic acid (NAA) at four level, A:Zero B: 0.01 mg1-1 C:0.05 mg1-1 D:0.1 mg1-1. Analysis of variance was performed on the collected data, and mean comparison was carried out, using Duncan's multiple range test (p<0.05). Results showed that light period increased the total weight, number of bulblets and regeneration percentage, whereas darkness treatment increased weight of bulblets and rooting percentage. Number and weight of bulblets, regeneration percentage and number of roots was better when explant obtained from basal scale. Different concentration of BAP had significant effect on some traits. The highest number of bulblets, rooting and regeneration percentage obtained in control treatment and the greatest weight of bulblets obtained by 0.1 mg1-1 BAP and the highest number of roots obtained in control and 0.01 mg1-1 BAP. Different concentration of NAA had also significant effects on some traits. The greatest total weight and number of bulblets obtained by 0.1 mg1-1 NAA and the weight of bulblets increased in all of the NAA concentrations. Concentrations of 0.05 and 0.1 mg1-1 NAA increased number of roots and rooting percentage.