Evaluation of interaction of Corticosterone and verapamil on anxiety related behavior in mice

Many evidences indicated that activation of glucocorticoids receptor has modulatory effects on anxiety and probably activation of calcum channel interaction with these effects. Therefore, aim of this study, was determine of interaction of corticosterone and verapamil on modulation anxiety in elevated plus maze in mice. In this experimental study 110 adult male mice (25-30 g) were used. In experiment 1: Saline + Corticosterone (0.5, 1, 3 and 10 mg/kg, IP) or vehicle (ethanol 2%+Saline) were injected 30 min before of evaluation anxiety. Also in experiment 2: Verapamil (5, 10 and 20 mg/kg) were injected 10 min before of Corticosterone (1 mg) or vehicle. Results indicated that corticosterone at doses of 1 and 3 mg/kg significantly reduced anxiety reaction in mice (P<0.01). Also verapamil in doses of 5 and 10mg/kg modulate of Corticosterone effecs. Finding of this study indicated that Corticosterone decrease of anxiety reation. Also verapamil modulate of effect of corticosterone, if injected before of that. Therefore one of mechanisem that probably involve in anti anxiety of Corticosterone is activation of calcium channel.