Antinociceptive and anti-inflammatory effects of hydroalcoholic extract of Vitex agnus castus fruit in mice

Anti-inflammatory and antinociceptive effects of Vitex agnus castus has been mentioned in traditional herbal medicine. In present study we decided to evaluate the effects of anti-inflammatory and antinociceptive of Vitex hydro-alcoholic extract in mice. In this experimental research 112 adult male NMRI mice were used. Mice were divided into 7 groups for inflammatory test: first group was control. The second group was positive control group (receiving dexamethasone15 mg/kg) and the other five groups received different doses of hydroalcoholic extract of Vitex fruit (265 365 465 565 and 665 mg/kg i.p.). Inflammation was induced by xylene in ear of mice. Formalin test was used for evaluation of antinociceptive effect of extract. In this test mice were divided into 7 groups: control morphin (10mg/kg) as positive control group and Vitex extract groups (265 365 465 565 and 665 mg/kg). All drugs were administered i.p. 30 min before each test. The data were analyzed using one-way ANOVA followed by Tukey-kramer multiple comparison test. Results showed significant anti-inflammatory effects of extract at all doses as compared with control (P