Synergistic effect of rCagA and LPS of H. pylori O2 serotype in induction of proper immune response against H. pylori

H. pylori is one of gastric bacterial infectious agents that reported from world whole. This organism is heterogen and have hyper variable regions in its genome that to cause organism escape from immune response. H. pylori cagA+ strains will report that is stimulating factor for adenocarcinoma, gastritis in infected persons. H. pylori LPS have less toxicity, mitogenicity and pyrogenicity than Entrobactericeae. In this study we investigated the LPS and rCagA senergistic effect on stimulation of Th1 immune response in mice model.

LPS of H. pylori O2 serotype was extracted by hot phenol-water method. Proper conserved fragment of cagA was expressed in proper vectors. These antigens were injected to Balb/c mice and immune response was assayed by ELISA

The IgG1/IgG2a ratio in the immunized mice with rCagA and rCagA plus CpG was <1, indicating a Th1 type response, while the control group was >1, indicating a strong Th2 response. In mice immunized with LPS and rCagA, the immune response elevated which indicated synergistic effect of this antigen on stimulating of strong immune response against H. pylori infection

Effective immunizations against H. pylori will possible affected treatment in next future. Protective immune response in H. pylori is balance between Th1/Th2.These data suggest that immunization with rCagA and LPS promoted a Th1 immune response. H. pylori rCagA and LPS serve as an excellent antigen for immunization. In conclusion, we recommended multicomponent vaccine contain of rCagA and LPS for vaccine formulation against H. pylori infection.