Determination of CMV infection in CSF of children with meningoencephalitis: PCR method

In recent years, many ill cases with cytomegalovirus reactivation in non-immuno compromised persons reported. Goal of study: to determine the CMV infection in cerebrospinal fluid of aseptic meningoencephalitis children hospitalized in Rasul & Mofid hospital (2005-2007). In a cross sectional study 132 cases selected with simple sampling. CMV-DNA in their Cerebro spinal fluids searched by qualitative PCR. The age range of the study patients was 5 month- 13 years, median age= 2±3.7 years; 87(65.9%) male and 45(34.1%) was female. The presenting signs and symptoms were convulsion 77(69.4%); meningitis 25(18.8%), loss of consciousness 47(37%); neurologic defects 15.9%. DNA extrated in 11 cases.