Identification prevalence of Bovine Leukemia Virus (BLV) in Holstein Dairy Cattle by DNATest

Bovine Leukosis that is established by leukemia virus. an exogenous C-type oncovirus in the Retroviridae family, is one of the diseases that can infect various animal specieses naturally or experimentally. In order to investigate the prevalence of Bovine Leukemia Virus (BLV), blood samples were taken from 175 Holstein of dairy cattle. Genomic DNA was extracted from 100? l of blood samples. Gel monitoring and spectrophotometric methods were used to determination quantity and quality of DNA. In standard PCR reactions primers gag1 and gag2 amplified a 347 bp fragment from the polymorphic region of BLV gene. Products of amplification were recognized by electrophoresis on 1.4% agarose gel stained with ethidium bromide.48(27.4%) cattle were positive.