Assessment of the expression of self renewal genes (Tcl1, Tbx3, Dppa4 and Esrrb) in bladder, Liver, colon and prostate cancers

Here we examined the expression of self renewal regulatory factors such as, Esrrb, Tcl1, Tbx3 and Dppa4 in several tissue samples of cancers and cancer cell lines. These genes are required for efficient self renewal of embryonic stem cells.

Caco2, HT-29, HT1376, Ln Cap, and HepG2 cells were cultured in T25 flasks. Considering the clinical and laboratory findings, human tumor samples were obtained under direct supervision of the medical specialists. Then we evaluated expression of self renewal genes (Tbx3, Tcl1, Esrrb, Dppa4) by reverse transcriptase polymerase chain reaction (RT-PCR) in the above mentioned cells and human tumor samples. To confirm the validity of the laboratory tests, we studied negative control samples and internal control genes.

Our data revealed the expression of self renewal genes (TCL1, TBX3, ESRRB and DPPA4) in bladder, liver, prostate and colon cancers and also cancer cell lines.

Colon, liver, prostate and bladder cancer cells can express TCL1, TBX3, ESRRB and DPPA4 genes, which are specific markers of stem cells. Therefore in malignant cells of the above mentioned cancers, some cells have the characteristics of stem cells and can play an essential role in the proliferation of malignant cells.