THE 4G/5G GENETIC VARIATION IN THE PROMOTER OF PLASMINOGEN ACTIVATOR INHIBITOR-1 (PAI-1) GENE IN PATIENTS WITH RECURRENT MISCARRIAGE AND HEALTHY CONTROLS

Deletion/insertion of a single guanosine in the promoter of the plasminogen activator inhibitor-1 (PAI-1) gene, resulting in two alleles containing either 4 or 5 guanosines (G). Results of recent studies showed that presence of PAI-1 4G allele may increase the risk of human disease. The aim of present study was to determine the 4G/5G genetic variation in the promoter of PAI-1 gene in patients with recurrent miscarriage and healthy controls. Genotypes of 45 cases with recurrent miscarriage (at least 3 unexplained abortions) and 64 controls were determined by RFLP-PCR. The findings of present study shows: Frequency (percent) of PAI-1 4G allele in cases and controls were 29(32.22%) and47(36.72%), respectively; frequency (percent) of PAI-1 5G allele in cases and controls were 61(67.78%) and 81(63.28%), respectively. Frequency (percent) of PAI-1 5G/5G and 4G/4G was 16(35.56%) and 29(64.44%) in cases and 17(26.56%) and 47(73.44%) in controls, respectively. PAI-1 4G/4G genotype was not observed in studied groups. Statistical analysis showed that the frequencies of PAI-1 -675 4G/5G alleles and genotypes did not significantly differ between cases and controls. The findings of present study show that it is not possible to find any association between PAI-1 -675 4G allele and predisposition to recurrent miscarriage in tested population.