Histopathologic Effect of Celecoxib on Primary Intention Healing of Stomach Surgical Wounds in Rats

Synthetic drugs are more effective substances in improvement of wounds healing. The main objective of this study was to histopathologically evaluate the effect of celecoxib on primary intention healing of stomach surgical wounds in rats. Male Wistar rats were randomly assigned into three groups; sham, negative control and experimental groups. A gastrotomy incision with the length of 1 cm was made in greater curvature of the stomach of the rats, and then was sutured in two layers. Experimental group was treated with oral celecoxib (15 mg/kg solved in 10 ml/kg dimethyl sulfoxide 5%) daily for 15 days. Negative control and sham groups received dimethyl sulfoxide 5% and normal saline (10 ml/kg), respectively, in the same manner. Histopathological studies for evaluation of healing were carried out in euthanized rats using H&E and Masson's trichrome staining methods. The numbers of fibroblasts and capillary buds in the experimental rats were significantly more than those in the sham and negative control groups (P<0.05). Epithelial gap in the experimental rats was smaller than that in the two other groups (P<0.05). There were significant differences of collagen contents in the region of wound healing between the experimental and two other groups (P<0.05). Differences between the sham and negative control groups of the mentioned factors were not significant. The results of this study indicate that celecoxib shows a beneficial effect on the surgical wound healing of stomach in the rats.