Amplification and flagellin typing of pseudomonas aeruginosa

Pseudomonas aeruginosa possesses a polar flagellum made up of flagellin subunits that encoded by fliC gene. Flagellin from several strains of P. aeruginosa on the basis of reaction with polyclonal antibodies and apparent molecular weight was divided into a or b types. After extraction of the genomic DNA from two standard strains 8821M (Type a) and PAO1 (Type b) and some clinical strains of P. aeruginosa, the full coding sequence of fliC was amplified by polymerase chain reaction (PCR) using the designed specific primers and its fragments length was compared. The PCR and sequencing results showed that the length of fliC genes from the a and b strains were 1160 bp and 1460 bp respectively. This method could be utilized to determine flagellated (Motile) and non-flagellated strains of P. aeruginosa, genotyping, amplification of full coding sequence of fliC gene in order to clone and express recombinant flagellin protein.