Assessment of genetic diversity of Holstein and native cattle in Kerman province using Inter Simple Sequence Repeats

Alarge number of marker protocols that are rapid and require only small quantities of DNAhave been developed. Sampling was carried out on 120 Holstein cattle from 4 herds in Kermancity and 80 native cattle from 5 herds in Bam city. Both ISSR primers tested, (AG)9C and (GA)9C, produced many different bands. Based on results of this study, ISSR markers produce numerous comparable bands and ISSR fingerprinting patterns are highly heritable. Polymorphisms were abundant among 9 herds tested with these primers, (AG)9C primer gives the more informative profiles than (GA)9C and comparison of ISSR profiles can be successfully applied to study difference in intra and interpopulations of cattle. Mean of gene diversity for (AG)9C marker in native and Holstein herds was 0.57and 0.28 and for (GA)9C marker was 0.35 and 0.15 respectively. Mean of polymorphic loci number and mean of polymorphic percentage in any population based on (AG)9C marker for native herds were 15.60 and 44.65 and for Holstein herds were 17.75 and 54.29 respectively and based on (GA)9C marker for native herds were 8.40 and 70.01 and for Holstein herds were 10.25 and 85.42 respectively. The highest of polymorphic percentage based on (AG)9C marker in Holstein and native herds was 74.29% and65.71% and based on (GA)9C marker was 100% and 91.70% respectively. Thus, ISSR markers are a good choice for DNA fingerprinting of cattle.