Exon 2 of BMP15 gene polymorphismin Jabal Barez Red Goat

Bone morphogenetic protein 15 (BMP15) is member of the transforming growth factor-beta superfamily that has crucial roles in fecundity of goat and sheep. Previous investigations confirmed that the fecundity mutations of sheep presented in goat. Different mutations in the bone morphogenetic protein-15 (BMP-15) gene has increased ovulation rate and infertility in sheep. To illuminate polymorphisms in BMP15 gene, was characterized the coding region of BMP15. Two exons (1 and 2) encoded prepropeptide of 394 amino acids in BMP15. Blood sample collected randomly from 100 goats from Red Jabalbarez goat from 10 farms and genomic DNA was isolated using the DNA kit. Two primer pairs were used to amplify genomic DNAof Bmp15 gene, and the PCR products were separated on agarose gels. The PCR products were electrophoresed and visualized by Ethidim Bromide staining. The results showed that amplication products had good specificity, which could be directly analyzed. Single nucleotide polymorphism of FecXB and FecXG loci in BMP-15 gene were determined using PCR-RFLP technique. In this study, there was no evidence of mutation in FecXB and FecXG in these goats, all of which were monomorph for exon 2 of BMP-15 gene. Further investigation should be directed at other loci of BMP-15 gene or other genes, using larger sample sizes.