Effect of Different Ratio of Trypsin Enzyme, Times and Temperatures on Protein Recovery of Viscera Yellow Fin Tuna (Thunus albacores)

In present paper the optimum extraction condition of protein of Hydrolysate from the viscera of yellow fin tuna (Thunus albacores) were studied. Enzyme hydrolysis has been performed at enzyme to substrate ratio: 1.3-3.7 mg/gr.protein, time of reaction: 1-10 hours, temperature: 30-44 ºC and using RSM method and central composite design(CCD) with 4 repetitions at central point and five levels of each treatment. Results have been compared with average at statistical level where α = 0.05. According to results,the protein recovery increases with an increase in enzyme activity from 1.3 to 2.5 mg When enzyme activity increases to 3.35 mg, the protein recovery reaches a constant level and then decreases with an extra increase of enzyme amount to 3.7 mg.The rate of protein recovery increases, With an increase in temperature from 30 ºC to 37 ºC,And then the enhancement of recovery rate decreases with more increase of temperature from 37ºC to 42ºC, However it will remain constant for temperatures beyond 42ºC.The protein recovery decreases With increasing the hydrolysis time between 60 to 138 min. and it reaches a stationary phase somehow during 138 to 330 min.In optimum point of hydrolysis, protein recovery was 75%. This condition were observed in enzyme ratio of 2.41 mg, Temperature of 38.75ºC,and hydrolysis time of 570 min. Results showed that protein recovery is mainly influenced by ratio of enzyme to substrate rate and temperature, while time of hydrolysis should be considered as a function of temperature and enzymatic ratio.