A phylogenetic study of Tangsayad and Karayi wild sheep (Ovis orientalis) populations

The phylogenetic study of Tangsayad and Kerayi wild sheep populations was conducted by using 5 microsatellite markers (BM6444, MCM63, MCMA2, LSCV38, and OARAE129). The PCR responses for all 5 markers were done truly and each marker was checked. The OARAE129, LSCV38, MCM63 markers showed a meaningful deviation from Hardy-Weinberg equilibrium in wild sheep populations, and 60% of Poly Morf locus which contains 3 LSCV38, MCM63, OARAE129 markers, and the two MCMA2 and BM6444 markers were Mono Morf. Low average of Allel for any locus (1.6 Allel) showed the low genetic diversity of wild sheep. Nie (1972, 1978) indicators were used to obtain the genetic distance between two wild sheep populations, which showed the high genetic similarity (0.9615) and (0.9771) and low genetic distance (0.0392) and (0.0232) in Tangsayad and Karayi wild sheep populations. Observing the average heterozygosity showed that life and the future of these animals are in danger.