Quantitative and qualitative study of phenolic compounds and antioxidant activity of plant pulicaria gnaphalodes

Plants are almost rich sources for phenolic compounds such as flavonoids, tannins and anthocyanins, which are the most important natural antioxidants. These diet antioxidants are important as they protect human body against oxidative stress and therefore maintain appropriate health. The purpose of this study is to evaluate quantitative and qualitative assessment and antioxidant activity of phenolic compounds in Pulicaria gnaphalodes as diet synthetic antioxidant substitutes. In this study, firstly the plant phytochemical investigation was conducted. Then total phenolic contents and total flavonoid contents were determined using spectrophotometery. The antioxidant activity (AOA) of extracts were evaluated with 2,2-diphenyl-1-picryl hydrazyl (DPPH) method and the results were compared with synthetic antioxidant BHT. The results showed that total phenolic contents, in water, ethanol and methanol were extracted 27.2, 26, 25.91 mg of GAE / g of dry sample respectively and also total flavonoid contents in water, ethanol and methanol were extracted 25.12, 21.32, 24.97mg QE / g of dry sample in due order. IC50 of Pulicaria gnaphalodes ether extract was 9/9 ± 0/20 μg / ml. The phytochemical results indicated the presence of flavonoids, tannins and anthocyanins in total extract. Regarded to the above results, it could be concluded that the extracts of Pulicaria gnaphalodes have contain high amounts of phenolic compounds and antioxidant activity. Therefore, this plant can be a potential source of antioxidant compounds for food and pharmaceutical industry.