Differential polypeptide expression olive plantlet under osmotic stress

For study of olive plantlet response under osmotic stress condition، in the first step embryos of the olive cv. Zard were cultured on solid 1/2MS media، after one month، half of the green plantlets were transferred to the solid 1/2MS containing 170 mM Nacl، the plantlets were sub-cultured، after two weak، according to different components of olive plantlets، several methods used to extract total proteins from both plantlets under stress condition and non stress condition. Equal quantity of total protein from both two samples was subjected to 2DE and protein profile of stressed plantlets was compared to non-stressed plantlets. Up and down regulated of polypeptides were interpreted as response of plantlet to the induced stress and the difference in polypeptides profile of plantlet under stress versus control plantlet was also considered. Significant difference were up regulation of three polypeptides and down regulation of three polypeptides.