Studying of glutenin subunits diversity in Aegilops tauschii accessions by SDS-PAGE method

Aegilops tauschii is a goatgrass, diploid and autogame species that is origion of D genome in Triticum aestivum. In this research, we studied high molecular weight of glutenins (HMW-GS) in 28 populations of Aegilops tauschii that collected from different provinces of Iran and a cultivar of bread wheat (Chinese spring) as control. Laemeli et al (1970) method was used for extraction of storage protein, then SDS-PAGE was used for separation of subunits of glutenins. After coomasssie blue and silver staining methods, we scored and analyzed the migration of bands.We calculated similarity matrix using jaccard coefficient and used cluster analysis based on UPGMA algorithm. In coomasssie blue method, similarity coefficients varied from 0.42 to 1; these populations were clustered and Chinese spring located in a single group. In silver staining method similarity coefficients varied from 0.57 to 0.95. We evaluated quality of protein bands in bread making quality based on Yan et al (2003) method. The accessions were not consistent with their geographical regions. Also,we suggested coomasssie blue method for staining of protein gels in order to detection of genetic diversity.