Effect of Tamoxifen on the uterus tissue,estradiol and LH in female Wistar rats

Tamoxifen is a nonsteroidal antiestrogen which is prescribed for eatment f breast cancer.Some negative effects of this drug on the reproductive system has been observed. The major aim of this study is to investigate the effect of Tamoxifen on the uterus histological change,estradiol and LH concentration in the females Wistar rats. Three group of rats (250 gr range) gavaged with Tamoxifen doses of 200, 400 and 600 μg/kg B.W(exp.1, exp.2, exp.3) dissolved in solvent (ethanol 60 %and hysiological solution) for 30 days. The sham group was gavaged with solvent and control did not receive olvent and drug. On days 1and 30 after the end of treatment, blood hormones such as estradiol and LH concentration were measured with RIA method, also sections of uterus stained with H &E method and were observed histologically. Results of the observation were nalyzed by the NOVA- oneway,Tukey test and using SPSS software determined ±SE the means Result were termed ignificant if P≤0.05.In the first sampling estradiol, LH concentration and the diameter and thickness of uterus layers (perimetrium, myometrium, endometrium) were decreased decreased significantly in xperimental groups which received tamoxifen compared with control group (p<0.001).The diameter and thickness of uterus layers (perimetrium, myometrium, endometrium). In the second sampling, the estradiol and LH concentration, the diameter and thickness of uterus in perimental groups, increased compared with first sampling. The number of offsprings in experimental groups were less than control litters. These findings suggest that Tamoxifendecreases the fertilization ability and has egative effects on uterus tissue and estradiol and LH concentration steroid hormons in female Wistar rats.Some of negative effects of Tamoxifen on reproduction ability, is abolished with time elapsed.