Detection of Experimental Cryptosporidiosis in Neonatal Mice and Rats by Nested-PCR
Author(s):
Abstract:
Purpose
The purpose of this study is to model Cryptosporidiosis in laboratory animals. The parasites were inoculated into animalsand thenmultiplied. The process of proliferation was compared to controlCryptosporidiosis in humans.Materials And Methods
Twenty-five laboratory mice (4-7 days of age) and twenty-five laboratory rats (5 days of age) were assigned to the category I while the category II (control group) consisted oftwenty-fiverats and twenty-five mice. The two categories were infected with 5×105 Cryptosporidium parvum oocysts originated from a calf by using a 24-gauge & 20 gauge ball-point feeding needle. On 4-9 days of post inoculation the intestine, colon, and rectum were removed. Cryptosporidium infection was determined by detecting oocysts in intestinal homogenates by Staining and PCR method. Simple extraction and purification method was used by ficoll gradient centrifugation. Also, twenty laboratory rats (4-6 weeks of age) were intramuscularly injected with dexamethasone(Sigma, Chemical Co. UK) two times per week, and the last injection was given with 5×105 Cryptosporidium parvum oocyst on the same day as oral inoculation. The water was supplemented with tetracycline to avoiding secondary infections.Results
Two to four million purified oocysts with a maximum of 10 million were routinely obtained per mouse and rat. Also the day in which oocyst excretion is the highest was determined. The number of oocyst per neonatal mouse was (11±2)×105 on 9-12 days of post infection while similarly it was (10±1)×105 per neonatal rat.Conclusion
The evaluation of the cryptosporidiosis in immunocompromised animal models can help us to understand and control the Cryptosporidium infections.Keywords:
Language:
English
Published:
Annals of Military and Health Sciences Research, Volume:14 Issue: 2, Spring 2016
Pages:
70 to 74
magiran.com/p1579710
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!