Protective Effects of Hydroxytyrosol on Renal Ischemia-reperfusion Injury in Mice

Renal ischemia reperfusion (IR) is one of the most important causative mechanisms of acute kidney injury that is a major clinical problem occurs in some of the hospitalized patients. The aim of this study was to investigate the Effects of hydroxytyrosol (HT) on renal ischemia-reperfusion injury in mice.
Male mice were randomly assigned to three groups (n=9):1 Sham operated,2 renal IR (45 min ischemia followed by 24 h reperfusion),3 renal IR HT (5 mg/kg orally for 7 days before renal IR and one hour before IR). Animal were sacrificed and the blood and kidney tissue samples were collected for glutathione (GSH) and malondialdehyde (MDA) evaluation.
Renal IR injury led to decreases in renal tissue GSH and increases in MDA levels compared to sham operated. hydroxytyrosol partly inhibited the IR-induced decrease in GSH activity. In addition, in renal IR HT group, MDA was increased compared to renal IR group.
These findings suggest that HT diminished oxidative stress in renal tissue after renal IR.