Evaluating the Feasibility of Esophagotomy Suture Line Reinforcement Using Platelet Rich Fibrin Membrane and Its Effect on Wound Healing

T his study aimed to evaluate the feasibility of using platelet rich fibrin membrane as a novel on-lay patching biomaterial in canine esophagotomy and its effects on esophageal wound healing. Design-Experimental study.
Animals Eight adult mixed breed dogs of both sexes equally allocated to control and treatment groups.
Procedures- Longitudinal incisions measuring 3 cm were made in the cervical esophagus of all dogs (2 incisions in each dog). All incisions were sutured and on-lay patching was performed in four dogs using platelet rich fibrin. After 28 days, wound healing was assessed by macroscopic, histological and biochemical Methods Patching resulted in less adhesion formation (18.24 vs. 61.67 mm, p0.05). Histologically, platelet rich fibrin membrane mostly influenced wound healing in the outer layers of the esophagus particularly the muscular layer although a slightly better wound healing was observed overall.  Platelet rich fibrin membrane could be used as an alternative patching biomaterial in esophageal surgery although further investigations needs to be carried out particularly in clinical cases.