Superoxide Dismutase-Loaded Solid Lipid Nanoparticles Prepared by Cold Homogenization Method: Characterization and Permeation Study Through Burned Rat Skin
Message:
Abstract:
Background
Superoxide dismutase (SOD), which inhibits lipid peroxidation and scavengers oxygen radicals, is an effective enzyme for treatment of skin ulcer lesion especially due to burns. Superoxide Dismutase is a hydrophilic compound with high molecular weight and low affinity for partitioning into skin. Moreover, another important limitation for its use in medicine is thermal denaturation and inactivation..
Objectives
We developed solid lipid nanoparticles (SLN) dispersions for enhancing superoxide dismutase penetration across burned rat skin and enzyme protection against environmental degradation..
Methods
Solid lipid nanoparticles were prepared with cold homogenization method because SOD is a thermo sensitive compound. The characteristics of SLNs such as particle size, entrapment efficiency and enzyme release pattern and permeability through burned rat skin were evaluated..
Results
Solid lipid nanoparticles showed more than 90% entrapment efficiency and particle size lower than 102 nm. In vitro release study demonstrated SOD burst and sustained release characters in this manner with maximum of 65% of enzyme released after 48 hours. The SOD activity was measured and results indicated that SLN could protect activity of the enzyme. Differential scanning calorimetry of SLNs showed low crystalinity index percentage that is a reason for high entrapment efficiency and burst release character..
Conclusions
The results indicated that SOD-loaded in SLN was delivered into deep burned skin layer and induced high enzyme activity through the skin. Low particle size, application of lecithin as surfactant and low crystallinity index (CI) percentage were important factors for increasing SOD penetration through the burned rat skin. Percentage of activity by SLN dispersions through rat skin was 13 folds more than the control..
Language:
English
Published:
Jundishapur Journal of Natural Pharmaceutical Products, Volume:11 Issue: 4, Nov 2016
Page:
8
magiran.com/p1630553  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!