Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression
Author(s):
Abstract:
Objective(s)
Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage.Materials And Methods
For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW-1, IP) nicotine-received and high dose (0.4 mg/kg BW-1, IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining.Results
Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (PConclusion
Our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mRNA and protein levels of bcl-2 and up regulating the p53 and caspase-3 mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion.Language:
English
Published:
Iranian Journal of Basic Medical Sciences, Volume:20 Issue: 2, Feb 2017
Pages:
199 to 208
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