Effects of butyric acid and arsenic on isolated liver mitochondria and pancreatic islets of male mouse

Aim: The aim of present study was to evaluate the different doses of Butyric acid (BA) and Arsenic (As) on mouse liver mitochondria oxidative stress and pancreatic islets insulin secretion.
BA is found in many foods and As is a toxic metal in drinking water. They can induce oxidative stress in some tissue.
In this experimental study, Liver mitochondria were isolated by administration of different centrifugation method and pancreatic islets of mice were isolated by a collagenase method. Mitochondria by BA (35, 75, 150, 300 ?M) and As (20, 50, 100, 200 ?M) and the islets were incubated by BA (250, 500, 1000, 1500 ?M) and As (0, 50, 100, 200 ?M) for 1 hour. At the end of experiment mitochondrial viability and membrane potential, ROS, MDA, GSH and islets insulin secretion were measured by their specific methods.
BA and As administration increased mitochondrial levels of ROS, MDA and decreased GSH and pancreatic islet insulin secretion in a dose dependent manner (p Alone or in combination administration of BA and As induced oxidative stress in liver mitochondria and decreased insulin secretion of pancreatic islets.