Typing of Clostridium Perfringens Isolated from Broiler Chickens Using Multiplex PCR

Clostridium perfringens (C. perfringens) is related to gram-positive and anaerobic bacteria. This rod-shaped bacterium produces spores and can cause a wide spectrum of diseases in humans and animals. Every of five different bacterium types can lead to different diseases. Most of distinct bacterial recognition methods are not completely effective and practical in all condition. The objective of this study was to isolate and identify different species of clostridium from the intestinal tract of broilers in Kerman province; southeastern of Iran using multiplex PCR. For doing this research, the intestinal tract samples were taken randomly from 122 broilers chickens. Samples were purveyed and cultured and then morphology of created colonies was checked and gram stain examination was performed. Genus identification of these bacteria was performed biochemical tests. Then DNA extraction from isolated bacteria was carried out and then multiplex PCR with specific primers was performed to classify toxin. Toxin types and bacterial strains were identified according to synthesized PCR fragments length. The only type of bacteria that isolated from intestinal tract of broilers was type A of C. perfringens. Our results indicated that C. perfringens type A is the most common type in intestinal tract of studied broiler population.