Determination of Genotypes of Streptococcus Agalactiae Isolated from both Urine and Vagina of Pregnant Women Referred to Gynecology Clinics of Yazd, Iran -2015
Abstract:
Background And Aims
Group B streptococcus (GBS) may cause neonatal infection during and or after the delivery, and is the leading cause of sepsis, bacteremia, pneumonia and meningitis. The virulence factors are carried by both capsule and surface proteins by which serotypes and genotypes are determined. However, some genotypes are believed to be related in severity of neonatal diseases, therefor this investigation was aimed to determine the surface proteins genotype of detected GBS from both vagina and urine of pregnant women.
Materials And Methods
In the present study, a total of 346 vaginal and urine samples were obtained from the same pregnant women. Following culturing the samples on sheep blood agar medium and related tests (CAMP, Hiporate hydrolysis) the suspected colonies were further confirmed by polymerase chain reaction (PCR) technique. The detected GBS species were then genotyped using multiplex PCR assay.
Results
Out of 346 specimens, 57 (16.47%), and 33 isolates (9.5%) were identified as vaginal and urine GBS, respectively. Genotype rib was predominant in both vaginal discharge and urine specimens, by frequencies of 56.1% and 54.5%, respectively. Other genotypes of vaginal GBS were as epsilon (36.8%), anlpha-C (3.5%), and alp2/3 (3.5%); while GBS in urine were as epsilon (36.4%), alp2/3 (6.1%), and anlpha-C (3%).
Conclusions
The prevalence of GBS in both vaginal and urine samples were reliable in comparison to other societies. These results showed that genotype rib of GBS, which seems to be associated to neonatal diseases, is dominant genotype in both urine and vaginal samples.
Language:
English
Published:
International Journal of Medical Laboratory, Volume:4 Issue: 3, Aug 2017
Pages:
180 to 188
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