Effect of Staphylococcus Aureus and Streptococcus beta-haemolytic Supernatant's on Leishmania Major Promastigotes Viability: An In Vitro Study
Author(s):
Abstract:
Background And Aims
Leishmaniasis is an intracellular protozoan- parasitic disease, the common vector of transmission. Both zoonotic and anthroponotic cutaneous leishmaniasis (CL) are endemic in different foci. With regard to the cutaneous form, 1.0-1.5 million cases were reported annually with 90% of the cases. Although antimony-containing compounds that are the main drugs used to treat Leishmaniasis has been recommended for CL treatment by the World Health Organization, but there are some restrictions in this case, including high expense, side effects, frequent injections need, and incomplete efficacy. The current research was conducted the effect of Staphylococcus aureus and Streptococcus beta-haemolytic Supernatants on Leishmania major promastigotes (PMs) viability: an in vitro study.Materials And Methods
Staphylococcus aureus and Streptococcus beta-haemolytic cultured for preparing supernatant, then Leishmania (L) major strain [MRHO/IR/75/ER] PMs cultured in Novy-Nicolle-Mac Neal (NNN) and roswell park memorial institute 1640 media. The cell proliferation of enzyme-linked immunosorbent assay, BrdU (Chemiluminescent) was performed as described by Roche Diagnostics. The mean of the viability PMs of Leishmania (L) major strain [MRHO/IR/75/ER] in culture according to Staphylococcus aureus and Streptococcus beta-haemolytic supernatant, Glocantime concentrations and in the control group (Glocantime) was obtained.Results
It was shown that there was a statistical significant difference among Staphylococcus aureus and Streptococcus beta-haemolytic supernatant inhibits growth of Leishmania (L) major strain [MRHO/IR/75/ER] PMs with the control group (pConclusions
These exciting results suggest that Staphylococcus aureus and Streptococcus beta-haemolytic Supernatants have significant therapeutic potential as novel anti-leishmanial.Language:
English
Published:
International Journal of Medical Laboratory, Volume:4 Issue: 3, Aug 2017
Pages:
211 to 218
magiran.com/p1734679
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!