Enhanced Cutaneous Wound Healing by the Leaf Extract of Achillea eriophora D.C. Using the In Vitro Scratch Assay
Abstract:
Achillea eriophora D.C. (Asteraceae), an endemic species in Iran, is used extensively in Iranian folk medicine to treat various ailments, including gastrointestinal disorders, skin inflammations and wound healing. The present study was aimed to investigate the effects of the methanolic extract of A. eriophora leaves on the stimulation of human fibroblast cell proliferation and migration as two pivotal stages in wound healing process. Methanolic extract was prepared by maceration method. Total phenol and flavonoid contents were measured using spectrophotometry. Cytotoxic and proliferation effects of the extract were evaluated by MTT assay on human fibroblast cells. Moreover, migration of the treated cells was assessed by the closure of a denuded area, made by scratching on the confluent monolayer cultures. The results of cytotoxicity assay indicated that the methanolic extract did not have any cytotoxic effect on fibroblast cells when used at concentrations up to 2 µg/mL. Human fibroblast proliferation was stimulated by low concentrations of the extract (0.1-0.8 µg/mL), and the highest level of proliferation was observed in the lowest treatment (0.1 µg/mL). Migration of the cells was induced by intermediate concentrations (1-30 µg/mL) of the extract and the most effective dose was estimated at 1 µg/mL. Due to the fact that lower concentration of the extract, which showed the best proliferation and migration stimulatory effects, were not toxic on the human fibroblast cells, the crude methanolic extract from the leaves of Achillea eriophora could be recommended as a potential source for improving the wound healing activity in skin.
Language:
English
Published:
Journal of Sciences, Islamic Republic of Iran, Volume:28 Issue: 4, Autumn2017
Pages:
305 to 312
magiran.com/p1752364  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!