A Thoroughgoing Design of a Rapid-cycle Microfluidic Droplet-based PCR Device to Amplify Rare DNA Strands
Abstract:
DNA is a molecule and assortment of fruitful information of organisms and a wide range of viruses. Polymerase chain reaction (PCR) is a process used to amplify DNA strands in order to generate millions of them and extract the applicable information. Although conventional methods for PCR are flourishing to a certain extent, they have such major drawbacks as contamination, high material consumption, and low-speed function. By the combination of PCR devices with the microfluidic approach and integrating them with droplet generation technology, the mentioned problems can be eliminated. In this study, a novel two-step rapid-cycle droplet-based PCR (dPCR) device, considering the design of microchannel and heat transfer system, has been presented. First, numerous studies have been conducted to select the proper droplet generator for the integration of the droplet generation with the PCR device. Then, with the careful attention to the requirements of a PCR device, the geometry of different zones of the PCR device has been, meticulously, designed. In the next and last step, the heat transfer system for the designed zones of the PCR device has been planned. Afterward, results are examined carefully which indicate that in a cycle of PCR, they are not any major discrepancies between the designed dPCR and the ideal one—the one that is intended to be created.
Language:
English
Published:
Journal Of Applied Fluid Mechanics, Volume:11 Issue: 1, Jan-Feb 2018
Pages:
21 to 29
magiran.com/p1779177  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!