Bioprospecting and molecular characterization of laccase producing bacteriafrom industrial contaminated sites

Laccases have vast prospective for biotechnological applications due to their outstanding bioremediation potential. These include abundant applications in effluent detoxification, enzymatic conversion of chemical intermediates, wine clarification degradation of textile dyes etc. In the present study, two potential microbes were isolated on solid medium containing guaiacol and ABTS for laccase activity out of 10 microbes. Two cultures PHP7 and PKD5 were selected for molecular characterization was carried out using 16S rRNA gene technology of PHP7 revealed as Bacillus cereus (KU878970.1).Partial amplification of laccase gene contain conserved domain of multicopper oxidase family. The biomass produced by PHP7 was 0.053 mg/5 mL, while PKD5 was 0.058 mg/5 mL. While dye degradation of PHP7dye of 64.28% after incubation of 6 days at pH7 whereas PKD5 shows highest degradation of dye i.e. 61.90% after incubation of 8 days at pH8. PHP7 showed highest Laccase activity of 0.489 U/L at pH 7 while PKD5 showed 0.404 U/L Laccase activity at pH 8 at 8th day of incubation. Using laccase from PHP7 and PKD5 isolates, explored at industrial level for decolorization of coloured effluents that significance in environmentally friendly and play critical role as bioremediation at commercial scale. Bioprospecting and molecular characterization of laccase producing bacteriafrom industrial contaminated sites Two novel microbes (PHP7 and PKD5) isolated from different sample of industrial contaminated sites
After biochemical and molecular characterization, PHP7 and PKD5 isolate were nomenclature as Bacillus cereus and sequence was also submitted to NCBI (KP729612.1)
Molecular characterization of laccase gene from PHP7 isolate revealed as PHPBMCST laccase-like (cotA) gene submitted to NCBI(KX215765.1)
Bioinformatics approaches revealed presence of conserved domain of cupredoxin super families of laccase protein
In partial amplified laccase gene, PHP7 and PKD5 isolates were also optimized for different pH and temperature for dye decolourization.