Antibacterial and Anti-biofilm Investigation of Electrospun PCL/gelatin/Lawsone Nano Fiber Scaffolds against Biofilm Producing Bacteria

The aim of this study was to evaluate anti bacterial and antibiofilm activity of PCL/gelatin nanofibers containing Lawsone against four pathogenic bacteria. Different concentrations of Lawsone (2-hydroxy-1, 4- naphthoquinone) (1, 3, 5 and 10%) were incorporated into polycaprolactone (PCL), gelatin (GEL) nanofibers via electrospinning technique. Presence of Lawsone in the scaffold confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial and antibiofilm activity of Lawsone loaded scaffolds against clinically isolated Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Proteus mirabilis were investigated by disc diffusion method. All scaffolds were able to inhibit the growth of S. aureus and MRSA. Additionally, PCL/GEL/Lawsone 10% had bactericidal efficacy against P. mirabilis. Obtained results of the biofilm formation assay revealed that PCL/GEL/Lawsone 10% inhibited the biofilm of S. aureus, MRSA and P. mirabilis by 56%, 32%, and 15%, respectively. Due to good antibacterial and antibiofilm activity of Lawsone containing scaffolds, PCL/GEL 10% can be a good candidate for application in wound dressing patches.