The complete genome sequence BK polyomavirus study in kidney transplanted patients
Message:
Article Type:
Research/Original Article (بدون رتبه معتبر)
Abstract:
Background
BK polyomavirus is a member of the Polyomaviridae. This virus has spread worldwide and up to 82% of the world populations are serologically positive. BK polyomavirus usually transmits through inhalation or fecal-oral way in childhood, as well as is likely to cause an asymptomatic disease. The virus can be reactivated in people who are immunocompromised.
Objectives
In this study we aimed to determine the complete genome sequence of BK polyomavirus in 5 patients receiving kidney transplantation in Golestan hospital, Ahvaz, south west of Iran.
Materials and Methods
The study was performed on urine and blood samples from kidney transplant ward, Golestan hospital, Ahvaz, Iran. To amplify the whole genome of BK polyomavirus AccuPower ProFi Taq PCR PreMix kit (Bioneer, South Korea) was used. Then, purified fragments were cloned into the vector. Sequences derived from the sequencing process were assembled by MEGA7 software. Data were analyzed using MEGA7 software and a phylogenetic tree was built based on the maximum likelihood ratio method.
Results
Overall, 40 urine samples (40%) and two plasma samples (12%) were positive for BK virus DNA. For the selected samples, W.G. Long PCR was performed and 5.1 kbp fragment was observed in all five samples. These fragments were cloned and sequenced. Genomic sequence analysis of the 5 strains studied showed 97.6% homology and all our study samples were of the same clade, which could be a reason for our patients to be infected with the same strain. Comparing the 5 isolates in our study with reference strains, showed more than 98% of homology, and variation was observed in less than 2% of nucleotides.
Conclusion
These five isolates showed more than 98% homology compared to the reference sequences, which can be attributed to the fact that these patients are infected with a common strain or that the genomic stability in the strain exists in our geographic area. We can conclude that in our geographic region there is a genomic stability and this strain can also be used as a positive control sample in other parts of the country
Language:
English
Published:
Journal of nephropathology, Volume:8 Issue: 2, Apr 2019
Page:
4
magiran.com/p1949724  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!