Detection of Toxoplasma gondii Antigens in Sera and Urine of Experimentally Infected Mice by Capture ELISA

Toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. The classical diagnosis of toxoplasmosis relies on serological methods. Detection of parasite or its components could be useful tool for early diag­nosis of the infection. Fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of T. gondii RH strain. Five of them sacrificed every day from day 1 up to day 7 post infection. Sera and urine of mice were tested by capture ELISA. T.gondii antigens were detected from 3rd and 2nd day in serum and urine, respectively, after infection until their death on day 7. Antigenemia detection of antigens of parasite was possible in a short period of acute infection with T. gondii by capture ELISA.