Journal of Herbmed Pharmacology
Volume:11 Issue: 3, Jul 2022

Ruta montana (RM) is a medicinal and aromatic plant (MAP) used in folk medicine, especially in North Africa, to treat digestive, infectious, respiratory, neurological, gynecological, and diabetic diseases. The current work aims to review the scientifically validated ethno-medicinal usage, bioactivities and phytochemistry of RM, in order to provide data support for further investigations. Data were procured from PubMed, Scopus, Google Scholar, Web of Science, ScienceDirect, and PubChem. The present study revealed that RM could be used to manage many diseases involved in public health problems, such as diabetes, hypertension, neurological disorders, infections, reproductive system disorders, and cancer. It might also replace chemical insecticides and fungicides since it exhibits antifungal, insecticidal, and larvicidal properties. RM extracts also contain mainly coumarins and alkaloids. The volatile oil of RM is characterized by an abundance of ketone compounds and 2-undecanone as major constituents. In the case of a high-dose administration, RM infusion can cause poisoning through the oral path. Thus, in-depth in vivo pharmacological studies and clinical trials are needed to transmute the traditional applications of RM into scientific-based information.

Withania somnifera belongs to the family of Solanaceae. It is widely used by the locals, especially in India, as a medicinal plant. W. somnifera is rich in alkaloid and steroidal lactone that account for various pharmacological activities. The present study aimed to review all the evidence on the ethnobotanical perspective of W. somnifera in some countries. This review also analyses the bioactive compounds that account for the pharmacological activities. The online medical literature databases such as ScienceDirect, PubMed, and Google Scholar were used to search articles up to March 2022. W. somnifera is widely used in Asian and African countries like India and South Africa, Pakistan, Egypt, Jordan, and Lesotho. In India, W. somnifera is prepared by boiling and crushing the leaves and roots to make a tonic poultice, juice, and paste to treat bacterial infections and bruises. Numerous in vivo and in vitro studies have demonstrated that W. somnifera exerts pharmacological effects such as anti-Parkinson’s, anti-Alzheimer’s, cardioprotective, antidiabetic, antiarthritic, and antibacterial properties. Withaferin A and withanolide are the major bioactive compounds contributing to the pharmacological effects. W. somnifera is a valuable plant that has been used in traditional medicine systems for a long time and is supported by its wide range of pharmacological activities. The extensive medicinal uses of W. somnifera are a sign of its great potential.

Stellaria media Linn., a member of the family Caryophyllaceae, is generally known by the name of Chickweed. This plant is extensively cultivated globally and is inherent to Africa, Asia, China, Europe, and North America. It is a well-known medicinal plant with immense therapeutic uses. Nutritional studies have revealed the presence of protein, especially 16 amino acids, vitamins, and minerals such as calcium, iron, phosphorus, and zinc. Phytochemicals, mainly flavonoids, isoflavonoids, saponins, tannins, alkaloids, phenolic acids, triterpenoids, phenolic compounds, and anthraquinone are present in chickweed. It has multiple therapeutic potentials like anti-obesity, anti-diabetic, anti-fungal, anti-bacterial, anti-inflammatory, anti-leishmanial, anti-anxiety, and toxicity profiles. The crude extracts and their metabolites did not show any toxicity in the experimental animal. This review summarizes the nutritional, phytochemical, pharmacological, and toxicity studies on this plant concerning its future use in pharmacological drugs.

Plants containing β-sitosterol and oleamide are important for various diseases. So, Dillenia indica, D. obovata, and D. pentagyna were investigated for phytochemicals, cytotoxicity, and genotoxicity levels on peripheral blood mononuclear cells (PBMCs) and Hela cells. The protective effect of D. pentagyna extract on a HepG2 cell line was also investigated.

Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were used for phytochemical analysis. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction (MTT) and comet assays were performed for toxicity testing and protective effects against DNA oxidative damage.

The major components were oleamide and β-sitosterol at 38.464-58.247% and 5.585-6.887% with concentration and quantity of β-sitosterol at 0.2-0.37 mg/mL and 0.42-0.964 mg/g leaf. The D. indica, D. obovata, and D. pentagyna toxicities on PBMCs showed IC50 values at >430, >430, and 350 μg/mL respectively, with no significant DNA damage (P > 0.05) compared to the negative control group. All plant extracts showed toxic activity on Hela cell with IC50 values at <0.43 μg/mL and induced significant DNA damage (P < 0.05) compared to the negative control group. Conversely, the activity of the D. pentagyna extract indicated low cytotoxic activity against HepG2 (IC50>430 μg/mL), no significant (P > 0.05) DNA damage induction, significantly (P < 0.05) decreased DNA damage level, and tremendous antioxidant effect. Additionally, a combined mixture of all plants in an equal proportion revealed no IC50 value and insignificant DNA damage.

All the studied species contained oleamide and β-sitosterol, with toxicity on Hela cells without toxicity on PBMC. The D. pentagyna species showed high antioxidant effects and no toxicity on HepG2.

Ganoderma resinaceum is used to treat oxidative and inflammatory-related diseases such as cardiovascular and liver diseases. Thus, this study aimed to evaluate the antioxidant and anti-inflammatory activities of different extracts from G. resinaceum fruiting bodies.

Aqueous crude (GRT), mycelial (MYC), exopolysaccharide (EPS I, EPS II), and water-soluble polysaccharide-rich (GRP I and GRP II) extracts of G. resinaceum were assessed for their free radical scavenging and metal chelating ions assays. The in vitro anti-inflammatory activity was evaluated by stabilization of erythrocytes’ membranes and protein denaturation assays. For the in vivo study, paw oedema was induced by administration of κ-carrageenan (0.1 mL; 1%) to male Wistar rats aged 4 to 6 weeks. Animals were pre-treated with G. resinaceum extracts (125 mg/kg) and diclofenac sodium (20 mg/kg). Inflammatory cytokine and chemokine levels were determined, and histological analysis of paw tissue was performed.

G. resinaceum polysaccharide-rich extracts (GRP I and GRP II) showed the best bioactivities. They scavenged DPPH (1,1-diphenyl-2-picrylhydrazyl, ABTS (2,2-azino-bis-3-ethylbenzylthiazoline-6-sulfonic acid, and NO (nitric oxide) radicals, and chelated ferrous ions, stabilized murine erythrocyte membranes, and inhibited protein denaturation. At 125 mg/kg, GRP I and GRP II restored the microarchitecture with a weak infiltration of immune cells in the subcutaneous tissues. Moreover, they decreased the overproduction of proinflammatory cytokines growth colony-stimulating factor (G-CSF), interferon gamma (IFNγ), tumour necrosis factor alpha (TNFα), chemokines (eotaxin, fractalkine), and increased the levels of anti-inflammatory cytokines (IL-10, IL-12p70).

G. resinaceum polysaccharide extracts could be potent antioxidant and anti-inflammatory agents.

Jicama (Pachyrhizus erosus, family Fabaceae) is a potent medicinal plant. Although extensive studies report the health benefits of jicama extract, few studies have investigated the efficacy of its dietary fiber in preventing metabolic diseases, including liver disease. The present study aimed to elucidate whether dietary fiber obtained from the jicama tuber counteracts the development of liver disease induced by high-sugar drinks.

Twenty-four adult male mice (DDY strain; 2 months old with bodyweight 22-25 g) were randomized into three groups: normal drink (ND), fed with tap water and standard chow; high-sucrose drink (HSD), fed with a high-sucrose drink and standard chow; and high-sucrose drink plus standard chow with 25% jicama fiber (HSD + JF 25%). After the mice were on their respective diets for ten weeks, the following parameters were measured: body weight, liver weight, malondialdehyde (MDA), histopathological alterations, blood glucose, and serum glutamate-pyruvate transaminase (SGPT).

Mice in the HSD + JF 25% group had significantly lower body weight (P < 0.01), liver weight (P<0.05), MDA (P<0.01), blood glucose (P<0.01), and SGPT (P<0.01) compared to those in the HSD group. They also had fewer histopathological alterations in the liver, as demonstrated by a lower proportion of degenerated cells and an overall lower histopathological score than those in the HSD group (P<0.05).

Adding jicama fiber (25% of standard chow) mitigates the increase in blood glucose and body weight and histopathological changes in the liver induced by high-sucrose drinks, showing liver protective activity.

Previously, we established the phytochemical composition and short-term administration safety of Guiera senegalensis (sabara), Cassia occidentalis (coffee senna), and Ziziphus mauritiana (jujube) leaves, which are common medicinal plants in Northern Nigeria. In the current study, heavy metal contents and long-term administration effects of the plants’ leaf extracts on hematological parameters and the kidneys of albino rats (Rattus norvegicus) were investigated. The heavy metals analyzed were copper, lead, cadmium, nickel, and manganese, while the hematological parameters evaluated were packed cell volume, hemoglobin, red blood cells, white blood cells, lymphocytes, and monocytes.

Twenty-four mixed-sex rats were distributed into four groups of six rats each. Group 1 was made the control, while groups 2, 3, and 4 were administered 1000 mg kg-1 one of the plants extracts for 90 days. Blood and kidney samples were collected across the groups for hematological and histopathological examinations.

The heavy metals were present in the extracts within the World Health Organization’s acceptable limits. The treated rats were anemic compared to the control. However, on average, only the C. occidentalis group showed significant differences (P<0.05) in hematological parameters. Unlike the control, the kidneys of the rats fed with Z. mauritiana and G. senegalensis showed vacuolation of cytoplasm and tubular degeneration, while the C. occidentalis-fed rats had inflammation and dilated Bowman’s capsules.

These findings reveal that constant administration of high doses of the extracts for a long time may cause health hazards. People are advised to seek an expert’s advice before using the plants.

Costus comosus is a potential medicinal plant used traditionally to treat various ailments. The present study aimed to evaluate antioxidant status, lipid peroxidation, and the ameliorative effect of its ethanolic leaf extract against diethylnitrosamine (DEN)-induced hepatocellular carcinoma (HCC) in rats.

HCC was induced by 0.01 % v/v DEN through the drinking water for 16 weeks. The animals were treated with ethanolic leaf extract of C. comosus (EECC) at 200 and 400 mg/kg for 16 weeks. In this study, tumour incidence, tumour volume, tumour burden, lipid peroxidation, antioxidant activity, liver marker enzymes, and histological responses were measured in the animals. At the end of the study, rats were sacrificed, their livers were removed and the levels of antioxidant enzymes were measured in the liver homogenate.

In DEN-treated animals, there were 100% tumour occurrences probably due to an imbalance in carcinogen metabolizing enzymes and cellular redox state. The oral administration of ethanolic leaf extract of C. comosus therapy at a dose of 200 and 400 mg/kg reduced lipid peroxide levels and restored the increased activities of liver marker enzymes and antioxidant status to near normal. The biochemical findings corroborate histological findings, indicating that the leaf extract has a significant hepatoprotective impact in a dose-dependent manner.

The results of the present study showed the promising anti-carcinogenic effects of ethanolic leaf extract of C. comosus against the DEN-induced HCC in rats.

Allium cepa extract has been reported to have anti-hypercholesterolemic activity in rats. This study was conducted to investigate the effects of standardized fermented A. cepa L. var aggregatum extract on cholesterol levels and HMG-CoA reductase enzyme.

The fermented A. cepa extract was standardized by the presence of quercetin using a validated high performance liquid chromatography (HPLC) method. The activity of the extract on HMG-CoA reductase was determined using HMG-CoA Assay kits, then measured by Nano spectrophotometry. In vivo study was conducted in hypercholesterolemic rats. The extract was administered orally at doses of 100, 200, and 300 mg/kg body weight (bw) to rats for 21 days and the cholesterol levels were measured every week.

All doses of fermented A. cepa extract and its marker compound, quercetin, ameliorated the levels of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) as compared to those of negative control (P<0.05). Of all the doses, fermented A. cepa extract at the dose of 200 mg/kg bw displayed the highest reduction in LDL-C levels. In addition, the extract at the dose of 200 mg/kg bw showed the strongest enhancement in HDL-C levels. The fermented A. cepa extract and quercetin also inhibited the HMG-CoA reductase enzyme with inhibitory activity of 61.78%.

The ethanol extract of fermented A. cepa shows anti-hypercholesterolemic activity. The strong anti-hypercholesterolemic activity of the extract might be due to the high amounts of quercetin, although other constituents may also contribute.

Essential oils are considered a potential alternative to synthetic drugs in the management of diseases such as peptic ulcer (PU) and ulcerative colitis (UC). This study is concerned with comparing the therapeutic effects of Cuminum cyminum L, Carum carvi L, and Thymus vulgaris L. essential oils on PU and UC models induced by ethanol.

Rats were divided into 10 groups; control groups were treated with saline and experimental groups with 500 mg/kg body weight of C. cyminum, C. carvi, or T. vulgaris essential oil. Curative effects were determined by measuring tissue oxidative markers, such as reduced glutathione (GSH), malondialdehyde (MDA), and myeloperoxidase (MPO), as well as the inflammatory marker prostaglandin E2 (PGE2), stomach pepsin (PEP), and colon alkaline phosphatase (ALP). Biochemical and histological examinations were done on stomach and colon tissues.

The current study proved the anti-ulcer effects of C. cyminum, C. carvi, and T. vulgaris essential oils. They improved the oxidative and inflammatory markers in both stomach and colon tissues and modulated stomach PEP and colon ALP activities. T. vulgaris essential oil modulated GSH and MDA levels resulting in a significant elevation in GSH levels by 120.43% and 99.46% and a significant reduction in MDA levels by 20.05% and 24.1% in PU and UC models, respectively. C. carvi essential oil was the most effective in restoring PGE2 by 71.51% compared to UC group. Results were confirmed by the morphological and histopathological changes.

C. cyminum, C. carvi, or T. vulgaris essential oils might be used in the management of acute PU and UC.

The extensive use of chemicals and antimicrobial agents in aquaculture has decreased the immune mechanisms of cultivated species and promoted the emergence of drug-resistant microorganisms leading to diseases among cultivated fish, affecting consumers’ health. Thus, the investigation of natural antibacterial and anti-stress agents is crucial. In the current study, we focused on the evaluation of the potential use of essential oils (EOs) as an antioxidant and antimicrobial agents in aquaculture.

The EOs, obtained by hydrodistillation from clove (Syzygium aromaticum), cinnamon (Cinnamomum verum), rosemary (Rosmarinus officinalis), artemisia (Artemisia herba-alba), cedarwood (Cedrus atlantica) and oregano (Origanum compactum) were analyzed by gas chromatography/mass spectrometry (GC/MS). Their antibacterial activities were carried out against five bacteria, pathogenic to fish in aquaculture, using the well diffusion and microatmosphere methods. The pathogens used were Vibrio anguillarum, Photobacterium damselae subsp damselae, Aeromonas salmonicida, Edwardsiella tarda, and Lactococcus garvieae. Then, the minimum inhibitory and bactericidal concentrations of each EO were determined. Furthermore, the antioxidant activity was performed in vitro.

The investigated EOs were effective against the pathogenic strains. They showed variable constituents such as phenols, sesquiterpenes, and monoterpenes. Regarding the antioxidant activity, cinnamon, clove, and oregano EOs showed their abilities to donate hydrogen to 2,2-diphenyl-1-picrylhydrazy (DPPH) radical and scavenge free radicals produced by 2,2-azino-bis-3-ethylbenzothiazoline6-sulfonic acid (ABTS), respectively.

These results gave insight into the potential use of phytobiotics in aquaculture as a safe strategy to substitute antibiotics to protect fish from oxidative stress and inhibit the emergence of drug-resistant bacteria for safer consumption of cultivated fish.

Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus.

Extraction was done using the cold maceration method. The minimum inhibitory concentrations (MIC) of the extracts were determined using the micro-dilution method. Swiss albino rats of 6 sub-groups with 6 animals each (36 animals/organism) were administered with 0.3 ml single oral dose of P. aeruginosa and S. aureus respectively. The animals received treatment for 5 days as follows: 0.5 ml of 5% dimethyl sulphoxide (DMSO) (negative control), 250 mg/kg of amoxicillin (positive control), 2 mg/kg of whole plant extract, 4 mg/kg of whole plant extract, 2 mg/kg of leaf extract, and 4 mg/kg of leaf extract, respectively. The packed cell volume (PCV) and white blood count (WBC) of the animals were determined before and after treatment with histology examination of vital organs.

MIC for S. aureus was 2 mg/mL; the mortality in S. aureus group at 2 mg/kg was 66.7%. The PCV values (50.5±0.5, 45.0±1.0, and 50.5±1.5) decreased after infection, and a corresponding increase in the PCV was observed after treatment with the extracts. Also, a significant increase in the WBC values (3.40±0.35, 4.10±0.15, and 3.30±0.40) following infection and a corresponding decrease after treatment were observed. Congestion of vessels in the kidney was also observed.

I. asarifolia has a dose-dependent antibacterial and curative activity, and could enhance innate immunity.

A cost-effective and ecologically friendly method of generating silver nanoparticles (AgNPs) includes pathways that utilize a variety of biological sources to decrease metal ions. This study was designed to synthesize AgNPs using a fungus strain Aspergillus flavus and evaluate its antibacterial activities alone or in combination with Foeniculum vulgare (fennel) essential oil (EO).

The antibacterial activity of different concentrations of biosynthesized AgNPs by Aspergillus flavus individually and in combination with fennel EO was investigated using disc diffusion methods and minimal inhibitory concentration (MIC). Bacterial species, including Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae, Shigella sp., Staphylococcus aureus, and Staphylococcus epidermidis were tested.

Formation of dark brown color, ultraviolet-visible (UV/Vis) spectroscopy, transmission electron microscope (TEM), and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) were used for the characterization of AgNPs. Obvious synergistic effects were observed between AgNPs and EO of fennel (F. vulgare) with all tested bacteria except S. aureus, through increases in fold area of inhibition (IFAs) within the range of 0.15 to 8.87. Although S. aureus had the most susceptibility toward both AgNPs and EO of fennel (24 and 17 mm, respectively), no synergistic activity was exhibited. The best synergistic capacity resulted from AgNPs and fennel EO was observed against S. epidermidis (8.87-fold in IFA).

This study revealed that when biosynthesized AgNPs were mixed with the EO of F. vulgare, they became more bacteriostatic and might be developed to treat bacterial infections in the future.

This study aims to evaluate in vitro and ex vivo antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx. root chloroformic extract against hydatid cyst protoscoleces and its effect on induction of apoptosis.

Various concentrations of the A. brachycalyx root chloroformic extract (56.25, 112.5, 225, and 450 mg/mL) were treated with hydatid cyst protoscoleces collected from the liver of infected sheep for 5-60 minutes in vitro and ex vivo. Eosin exclusion test was also utilized to measure the mortality of protoscoleces. Moreover, the extract effect was assessed on apoptosis induction in hydatid cyst protoscoleces by caspase-3 activity measurement.

The mortality rate of protoscoleces in in vitro was 100% after being exposed to 450 and 225 mg/mL of A. brachycalyx extract for 20 and 30 minutes and in ex vivo for 30 and 60 minutes, respectively. Following 48 h treatment of protoscoleces, A. brachycalyx chloroformic extract at the doses of 56.25, 112.5, 225, and 450 mg/mL, dose-dependently motivated the caspase-3 enzyme ranging from 8.8% to 29.6%.

A. brachycalyx root chloroformic extract had a significant protoscolicidal effect; however, extra surveys are required to assess its efficacy and safety as a promising protoscolicidal agent in clinical settings.

Zaringiah (Dracocephalum kotschyi) is a famous Iranian herbal plant with anti-inflammatory and spasmolytic activities. There is no standard drug dosage form for the D. kotschyi extract on the market. The objective of this project was to design a suitable oral dosage form for the hydroalcoholic extract of D. kotschyi.

Standard granules were prepared using the moist granulation technique. Physical properties of the granules were determined before filling the capsule with fixed doses of the drug (25 mg and 50 mg). Syrup was prepared in sucrose solution at 5 mg/mL concentration. Bioactivity and phytochemical assays were used for dosage form stability and uniformity evaluations before and after 3- and 6- months incubation. Pharmacological bioassay method was designed to determine the bioactivity of the products before and after incubation. Pharmacological effects of the prepared capsule and syrup were determined on rat isolated ileum and intestinal meal transit, respectively.

In this study, D. kotschyi extract was effectively formulated as capsule and syrup for oral consumption. Environmental and aging factors had no significant effect on the total flavonoid or phenolic contents or bioactivity of the manufactured products. Furthermore, the ingredients used in the formulation had no effect on the bioactivity of the active substances in the extract.

The standard oral dosage forms prepared from D. kotschyi extract can be used for clinical trials. In addition, we introduced a reliable bioassay technique, which might be applied for the evaluation of herbal medicines with antispasmodic activities.

Aqueous extract of Lagerstroemia speciosa (EALS) (Lythraceae) is widely used to treat diabetes. This plant has been shown an in vitro thrombolytic activity that indicates its potential to prevent the formation of blood clots in vivo. Thus, this study was undertaken to evaluate the antithrombotic and antihemolytic effects of EALS.

Rats of both sexes (200 ± 5 g) were divided into five groups of six animals. Each group received orally distilled water, EALS (250, 500, 1000 mg/kg), and acetylsalicylic acid (100 mg/kg) for five days. After treatment, the FeCl3-induced arterial thrombus formation method was used to determine occlusion time. A coagulometer was used to detect activated partial thromboplastin time (aPTT) and prothrombin time (PT). Rabbit blood was used to determine clot lysis activity in vitro and antihemolytic activity using the 2,2-azobis hydrochloride (2-methylpropionamidine) (AAPH) method.

EALS increased the occlusion time in a dose-dependent manner. At the dose of 1000 mg/kg, EALS increased the occlusion time significantly, from 4.59 ± 2.45 minutes to 15.52 ± 2.38 minutes (P < 0.01). At high concentrations (1-4 mg/mL), EALS showed a significant increase in aPPT and PT (P < 0.05). Streptokinase and EALS (4 mg/mL) induced significant clot lysis with percentage values of 78.48 ± 2.2 % and 49.5 ± 1.53 %, respectively (P < 0.001). EALS inhibited AAPH-induced hemolysis.

EALS exhibited antithrombotic and antihemolytic activities. The antithrombotic property of the plant could be attributed to its anticoagulant and thrombolytic activities. Regular consumption of L. speciosa leaves may prevent or treat thrombotic diseases.