فهرست مطالب آناتولی نیکلایویچ یوتوشنکوف

Our objective was to investigate the effect of glyphosate in induction of resistance to two plant bacterial pathogens. To do so, glyphosate at an optimal concentration of 1.8 mg / l was used on the transgenic potato, Odyssay cultivar, to induce resistance to two strains of pathogenic bacteria (21A of Pectobacterium atrosepticum and ENA49 of Dickeya dadantii). RT-PCR analysis on RNA isolated from transgenic plants, showed overexpression of aroA and potato defense response genes. Transgenic potato leaves infected with potato pathogenic bacteria, and then treated with glyphosate showed a high level of expression of pathogenesis-related genes (PR-2, PR-3, PR-5), especially PR-2 and defense response genes (HSR-203j, HIN1), especially HSR-203j. However; the plants infected with bacteria and non-treated by glyphosate did not significantly change the expression of these genes. The results showed that the treatment of plants by glyphosate may not only eliminate weeds of farmland but can also induces a systemic acquired resistance to pathogenic bacteria by expressing of PR proteins and defense response genes.

The treatment of plants by herbicide glyphosate can affect their resistance to disease. The effect of glyphosate on the expression of pathogenesis-related and resistance genes in transgenic potato infected with two strains of late blight pathogen, Phytophthora infestans was investigated. To do so, an optimal concentration of glyphosate (1.8 mg/L) were been used on transgenic potato, Vesnianka cultivar. a high level of expression of pathogenesis-related genes (especially PR-2) and defense response genes (especially HSR-203j). In these plants, no signs of burn and infection were observed were observed in the potato leaves infected with pathogen, and treated by glyphosate. The results showed that treatment by glyphosate could help to induce the resistance to P. infestans by expressing genes encoding defense response proteins.