فهرست مطالب احسان رخشنده رو

Establishment of experimental cystic echinococcosis (CE) in laboratory mice is required for the study of CE. Experimental CE may be established in primary or secondary forms; however, the primary form is more reliable for the study of CE.

The aim of the study was to assess the possibility of establishment of primary CE in Balb/c mice via the oral administration of Echinococcus granulosus s.l. eggs.

E. granulosus s.s. eggs were obtained from the gravid segments of the adult worms collected from an experimentally infected dog. Thirty-four female Balb/c mice were allocated into 4 groups and were orally infected with E. granulosus s.s. eggs as follows: 1) the mice with normal immunity, a single dose of 1000 eggs, 2) the mice with normal immunity, 3 doses of 500 eggs, 3) immunosuppressed mice, a single dose of 1000 eggs, and 4) immunosuppressed mice, 3 doses of 500 eggs. After 6.5 months, all the mice were opened and their internal organs were examined for the presence of CE cysts. The livers of infected mice were also examined for the presence of E. granulosus s.l. compartments by the PCR method.

There was no developed or developing CE cyst in the abdominal cavities or on the internal organs of all the mice in four groups. In addition, in the molecular study, all the examined liver samples were negative for the parasite material.

The results of the present study revealed that Balb/c mice are not a suitable host for establishment of primary CE following the oral administration of E. granulosus s.s. eggs.

In this study, the anthelmintic effects of the medicinal herb, Nigella Sativa L., was screened in vitro against the infective larvae of Parascaris equorum. Follow the collection of Parascaris worms, the egg content were depleted and hatched using sodium hypochlorite solution. The recovered larvae of the parasite were exposed to six concentrations (6.25, 12.5, 25, 50, 100 and 200 mg/mL) of the Nigella extract and then examined for the viability at 0, 10, 20, 30, 40, 50 and 60 minutes after the challenge. The results revealed that the concentration of 6.25 mg/mL had not any significant anthelmintic effects on P. equorum larvae. In contrast, the statistics indicated that all the higher concentrations of the Nigella extract remarkably reduced the viability of larva. These results were obvious during the first ten minutes of the experiment. With increase in time of exposure, the mortality effect of the extract elevated significantly. In addition, all larvae were inactivated after 40, 20 and 10 minutes after exposure at the concentrations of 50, 100 and 200 mg/ml, respectively. These results confirmed that this herbal extract possess good antiparasitic effects against P. equorum infections.