فهرست مطالب امین نکویی

The purpose of this study was to evaluate the effect of eight weeks continues training on the expression of miR29mRNA in the cardiac muscle of healthy male rats. Male Wistar rats (n=6/group) were randomly divided into two groups: sedentary and training group. Training was prescribed 70-75% vo2max for 30 min, 5days/week. Hearts were isolated 24 hours after the last session. Using quantitave RT-PCR, miR29 expression changes were analyzed then calculated by 2ΔΔСТ method. To determine the significance of variables between study groups, independent t-test was used. Results showed that there is a significant increase, in gene expression miR29a,c in training group compared to control group (P=0.001).But miR29b gene expression were significantly reduced (P=0.416). Based on the study findings, continuous endurance training induce physiological hypertrophy training is at the heart of the group and lead to a significant increase in expression levels miR29a,c. Since this increase leading to a significant reduction in expression of collagen isoforms, it can be concluded that rising miR29a,c in physiological hypertrophy induced by endurance training is directly regulates extracellular matrix and ventricular compliance.

The aim of the present study was to investigate the effect of HIIT run on PGC-1α gene expression in fast & slow twitch muscles of healthy male rats. For this purpose, 12 male Wistar rats divided into two groups: contorol (n=6) and exercise (n=6) , respectively. Intense interval training was consists of five days and a total of eight weeks, that was formed the three frequencies ( 90 to 100% VO2max intensity for four minutes and two minutes at an intensity of 50 to 60% VO2max). 24 hours after the last training session, extensor digitorum longus muscle (EDL) and soleus was extracted and relevant variables and expression levels were measured by RT-PCR. Results showed a HIIT run a significant increase PGC-1α gene expression in both muscle groups were trained (p=0/004 and p=0/001). According to the findings, implement HIIT will be increases PGC-1α and oxidative capacity in SOL and EDL muscles.

One of the important adaptations that occurs after exercise is increased capillary density or angiogenesis. Vascular endothelial growth factor, has a mitogenic role for endothelial cells and acts as an important intermediator in the process of angiogenesis. The aim of this study was to compare the effects of two kind of endurance training on vascular endothelial growth factor gene expression in healthy male rats. In this laboratory experimental study, 18 male Wistar rats at the age of eight weeks, with an average weight of 210/5± 9/77g were selected and randomely divided into three groups (control (n=6), ET (n=6) and HIIT (n=6)). Aerobic continuous training was performed 5 days a week, totally in eight weeks for 30 minutes with 70-75% VO2max and high intensity interval training consisted of three periods (four minutes with 90 to100% VO2max and two minutes with 50 to 60% VO2max). Vascular endothelial growth factor gene expression was measured by real time-PCR technique. To determine the significance of variables between these groups, one-way ANOVA and Tukey's post hoc tests were used. The results showed that the gene expression levels of vascular endothelial growth factor were increased significantly (p=0/006, F=7/243) in intense aerobic continuous and interval training groups compared to control group. Changes in exercise groups compared with each other were not significant. According to the results of this study, increased levels of vascular endothelial growth factor gene expression in both training groups caused pro-angiogenic function in endothelial cells and an increase in ratsVO2max following eight weeks training may be due to increased angiogenesis process. High intensity interval training may cause faster adaptations in the body of organism than aerobic continuous training.

Mir-210, is proangiogenic microRNAis end thelial cells. This microRNA, causes the repression of some genes and proteins target; so cause angiogenesis process. The purpose of this study was to determine the effect of a High Intensity Interval Training (HIIT) on Mir-210 and EphrinA3 receptor genes expression in soleusmusclesof male rats. In this experimental study, Twelve Wistar male rats(ageof eightweeks, average weight of 210/5±9/77)were randomly divided into exercise(n=6)and control (n=6) groups.High Intensity Interval Training was formed five days a week for eight weeks to taly including three Intervals (four minutes with an intensityof 90 to 100%VO2max and two minutes with an intensityof 50 to 60%VO2max).24 hours after exercise protocol, the rats were dissected and separated soleusmuscle. Mir-210 and EphrinA3receptor genes expression was performed by Real Time-PCRtechnique. Mir-210 and EphrinA3receptor genes expression were calculated by using the2∆∆CT and in dependentt-test to determine the significance of variables. Results showed that HIIT there had no significant effects on Mir-210 gene expression (p=0/16); Whe ars EphrinA3 gene expression in the exercise group was statistically significant (p=0/000). It seems that a non-significant increase of Mir-210 and reduce in EphrinA3 gene expres sion, causes proangiogenic Operation ofendothelial cells and an increase in VO2max of rats following eight weeks of HIIT performance can be due to increased angiogenesis process.