فهرست مطالب جمال رشیدیانی

Synthesis of homogeneous and equally important nanoparticles is very popular in various sciences. Preparation and use of gold nanoparticles is very important according to their applications in biotechnology. For synthesizing homogeneous gold nanoparticles many substrates and nanostructures have been used. This study aimed at synthesis of gold nanoparticles by apoferritin to be conjugated to antibody for detection of vibrio cholera.

In this study, gold nanoparticles were prepared in apoferritin and compared with apoferritin free gold nanoparticles. Data were characterized by UV-Vis, DLS, and FE-SEM spectroscopy.

Findings showed that the synthesized gold nanoparticles in apoferritin are more homogeneous.

The distribution of synthesizing gold nanoparticle in presence of apoferritin was so homogenous. In the following step, gold nanoparticles were separated from apoferritin and conjugated to recombinant antibody for detecting vibrio cholera. By bacteria aatchment on gold nanoparticles, red shift ocured on wavelength, which was also visible to naked eyes. So, gold nanoparticles could be considered as a colored biosensor for detection of vibrio cholera.

Aim and Pseudomonas aeruginosa is an important opportunistic pathogen that typically makes a biofilm. P. aeruginosa biofilms are intrinsically resistant to antimicrobial chemotherapies.The aim of this study was to evaluate the inhibitory effects of ethanolic extract of Malva sylvestris on the biofilm formation of Pseudomonas aeruginosa ATCC:27853 .
Material and The alcoholic extracts of Malva sylvestris which were collected from north western Iran(Kurdistan)were obtained by soxhlet apparatus. The antibacterial effect of plant extract was determined by well diffusion agar. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assessed by micro dilution method according to CLSL standard.
Finally biofilm formation of these this strain in presence of ethanolic extract of M. sylvestris was determined using modified micro titer plate method. Statistic analysis was done by t-test and analysis of variance(ANOVA) was used to compare means and data correlation.
The results showed a significant effect of ethanolic extract of M. sylvestris on the tested strain. The MIC and MBC were 62.5 mg ⁄ ml. The different concentration of ethanolic extract of M. sylvestris decreased significantly in biofilm formation. on P.aeruginosa in compared to gentamicin as the positive control (P value = 0.0124).
According to the finding Malva sylvestris, is effective in killing P. aeruginosa. bacterial biofim so this medicinal plant appears to be promising agent for prophylaxis against various pseudomonas infections

Cholera is a disabling infectious disease that is caused by Vibrio cholera O-1& O-139. The current procedures for the detection and enumeration of V. cholerae bacteria can often be costly in labor, materials, and time.The aim of this study was to evaluate the separation of V. cholerae O-1 using Immunomagnetic separation.

In this study, the polyclonal antibody against bacterial surface antigen (ompW) was immobilized on magnetic iron oxide nanoparticles and this approach was used to isolate bacteria from environment. Immobilizing process was applied using the 11-MUA in the presence of EDC and NHS. To confirm the immobilizing process, two methods (FTIR spectroscopy and Brad Ford proteinassy) were used. Bacterial strains from two medical centers of Tehran clinical samples were collected and identified using biochemical methods.

Functionalized iron oxide nanoparticles with antibody against ompW was successfuly accomplished. Nanoparticles were added to the environments containing bacteria and applying magnetic fields, the bacteria were isolated from the buffer. This method can isolate V. cholerae O-1 bacteria up to 2cfu from buffer samples. Culturing the bacteria attached to nanoparticles in specific media confirmed this isolation method.

The results showed that, in comparison to other separating methods, this technique is more powerful.

Cholera is an acute intestinal disease that is caused by some members of Vibrionasea family. Among more than a hundred family members, only Vibrio cholera O-1& O- 139 are pathogens for human and some marine animals. Surface proteins of these bacteria are immunogenic and act as colonization factor. The aim of this study was to evaluate the induction of rat antibody against surface proteins of Vibrio Cholerae O-1 (ompW).

The recombinant antigen was injected to rats according to the standard procedures. After four times of subcutaneous injection, the rat sera were collected and purified using protein G column chromatography. Then the efficiency of antibody response was determinedusing ELISA.

Recombinant protein concentration was measured to be about 0.9 μg/ml. Rat serum response to recombinant protein was positive up to the concentration of 1/25000 in ELISA reaction. Concentrations of purified antibody were estimated about 0.78 μg/ml. ELISA responses for antibody against ompW dealing with bacterial suspension with OD600= 0/5 was assessed positive to 1/25000 while they did not react with other intestinal bacteria.

The recombinant ompW protein was immunogenic and the produced antibody was able to identify whole Vibrio Cholerae O-1 bacteria and did not react with other related bacterial strains.