رقیه دریکوند

Proteins as direct products of genes are considered as the appropriate markers to assess genetic diversity and to identify resistant individuals. For investigation of Cypress (Cupressus sempervirens) seed storage protein based on quality activities by using SDS-PAGE method، 40 samples were taken on dried and healthy trees on two altitude classes. Results showed that weight of molecular bands on electrophoretic pattern of protein bands were between 18 and 125 KDa. Results of clustering and discriminant analysis also showed that trees from the two altitude classes could be distinguished by protein bands، but trees with different dried degrees could not be distinguished. Genetic diversity indices such as Shannon index and Nei، polymorphism percentage، showed trees with low and high dried degrees had the highest and lowest degree of the indices، respectively. Also trees from high altitudes had higher genetic diversity than trees from low altitudes. Diversity within the populations in the two altitude classes was more than variation between the populations. Also Genetic variation among altitudes in different populations (FST) was equal to 0. 42. Therefore، results implied that Cypress trees in the studied region contain high genetic diversity، especially at high altitude. Also we could not distinguish dried and healthy trees by using seed storage protein.

Cypress (Cupressus sempervirens L. var. horizontalis) is one of the native conifers in Iran. It is found in Tange Soulak Forest Reserve of Kohgiluyeh and Boyer-Ahmad Province، Iran. An experiment in a completely randomized design with factorial 4×2×2 was performed to assess the factors affecting Cupressus sempervirens seed germination. Dieback، slope، and altitude were analyzed at four، two، and two levels، respectively. Results showed slope to have the greatest effects on seed characteristics and germination. In fact، all studied parameters including 1000 kernel weight، humidity of the seeds، speed and rate of germination، and the mean number of seeds in a cone decreased with increasing slope. Moreover، tree diameter had a low positive relationship with seed weight. On the other hand، although the seeds collected from trees with high rate of dieback had the lowest mean speed and rate of germination; dieback had significant effects only on the mean germination time. There were significant positive correlations between 1000 kernel weight and all seed and germination parameters. Therefore، it is suggested that for conservation and regeneration of this species in the region، seeds should be collected from mother trees with a larger diameter and heavier seeds located on low-slope lands.