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The tissue reaction to stitch strings used in the intraoral surgery that can aggravate inflammation is an important issue. This study aimed to compare the tissue reaction formed around three types of stitches, including Demesilk (Demetech corporation,USA) that is not produced inside the country, Pedsilk and Suture, which all are natural nonabsorbable, on rats.
In this experimental study, 12 male rats with an approximate weight of 250 g were selected and divided into two equal groups. After anesthesia, the back hair of the animals was shaved and all three types of stitch strings were used differently in each sample. The first type of stitch was Pedsilk, the second type Suture, and the third one was Demesilk. In certain days (the second and seventh days), one group of the rats were sacrificed with a certain amount of fatal ketamine and all three types of stitch strings were removed separately and transmitted to laboratory and compared regarding inflammation, granulation and fibrosis formation. The data were analyzed using the Friedman and Wilcoxon tests with the SPPS software.
Statistical assessment carried out in all three types showed a significant difference among the strings in most cases. The manner of Pedsilk and Demesilk with regard to inflammation was similar in which the possibility of inflammation increased as the time passed and this possibility decreased in the Suture. The manner of both Pedsilk and Demesilk regarding the granulation tissue was similar in which the possibility of the formation of the tissue did not have a remarkable decrease as the time passed. In Suture, this possibility decreased remarkably. The manner of all three strings, Suture, Pedsilk and Demesilk, increased significantly regarding fibrosis tissue formation as the time passed (P The common use of stitch strings in intraoral surgeries and the fact that a stitch string is considered as an external body in a wound zone increases the risk of infection and inflammation in Demesilk and Pedsilk, but this possibility decreases in the Suture type. As a result, after the surgery, the use of the Suture in covering the surgery zone is supposed to be the most important factor in amending the tissue and the success of the surgery.

Non-steroidal anti-inflammatory drugs (NSAIDs) are drugs commonly prescribed in dental practice for the management of pain and swelling. But, rarely hypersensitivity reactions are reported.
Case Report: A 28 year old woman underwent periodontal plastic surgery (gingival graft). Postoperative analgesics (400 mg Gelofen ,oral) and antibiotics were administrated for the patient. Three hours after discharge of patient, she complained of redness, itching , rapid swelling of her eyes in 10 minutes, and watery eye discharge 1 hour after taking the drugs. She was treated with 8mg/2ml mg Dexamethasone IM at the dental department and with Hydrocortisone 100mg/ml IM and antihistamine drugs at the hospital.
There are no published protocols and sensitivity and specifity of skin pick testing and patch testing for Gelofen. So avoidance of re-exposure is the best management strategy. The use of Cox-2 specific medications would be a proper alternative for pain relief.

Introduction & In recent years stem cells have been evaluated for regenerating lost tissues. Therefore, many studies aimed to assess isolation of these cells from different origins, and culture them. The purpose of this study is to evaluate the osteogenic differentiation of stem cells derived from periodontal ligament of periodontitis affected teeth in melatonin contained media.
In this experimental-laboratory study after tooth extraction and isolating the apical segment of the roots, the periodontal ligament tissue was removed. Cells were expanded and after the third passage; flow cytometery analysis was performed to evaluate the surface markers (CD 105, CD146, CD90, CD 45, CD31, CD34, CD106 and CD 73). Adherent cell layer was used for osteogenic differentiation in melatonin contained osteogenic media. Then, osteogenic differentiation was evaluated using alizarin red staining, alkaline phosphatase and calcium content tests were performed.
Quantitative analysis of alizarin red staining on the 28th day demonstrated that mineralized nodule formation in the group supplemented with melatonin was higher than the control group. Results from alkaline phosphatase activity test on the 7th, 14th and 21st days demonstrated that, this activity was higher in the group supplemented with melatonin. Also, the amount of calcium on the 7th, 14th, 21st and 28th days, in melatonin group, was higher than the control group.
Melatonin (50 μM) may be beneficial for differentiation of PDL stem cells into osteoblast.

Introduction & After periodontal surgery the surgical site is usually covered with periodontal pack. It is suggested that dressings can reduce the rate of infection، bleeding and granulation tissue formation after surgery. In addition، they may promote tissue healing. The aim of this study is to evaluate periodontal dressing’s effect on wound healing، pain and bleeding after periodontal surgery. In this clinical trial study 33 patients attending the Hamadan dental school were evaluated. The patients need flap surgery on both sides of a jaw. Periodontal pack was used in one side of a jaw only. Inclusion criteria were: nearly similar disease distri-bution and planning modified Widman flap for both sides. Data were analyzed using SPSS16 software and chi-square and t-test. Average rate of pain in test and control groups was 1. 73 and 2. 79، respectively and the difference was statistically significant (P<0. 05) but the differences were not statistically significant regarding swelling، bleeding، gingival consistency، granulation tissue formation، gingival color and ease of eating، in the test and control group (P<0. 05). According the results of this study، periodontal pack may be beneficial in reduc-ing pain after surgery. (Sci J Hamadan Univ Med Sci 2014; 20 (4): 267-272)