سعیدرضا نورالهی فرد

Lucilia sericata is one of the facultative parasites which cause myiasis in animals and rarely in humans as an ectoparasite. Infestation in humans and domestic herbivorous animals occurs in wounds which was causes itching and inflammation. We report on a 4-year-old goat from Kerman, with signs of anorexia, progressive emaciation, decreasing mental clearness, and coma. From the wounds of her oral cavity, many live larvae were removed. The larvae proved to be L. sericata. This present report is the first report of oral myasis with osteolysis caused by L. sericata in goat.

Abortion in ruminants occurs for many reasons such that in developed countries despite the relatively complete diagnostic facilities the large number of abortions remains unknown. Most frequently isolated agents include: Brucella spp., Campylobacter spp., Leptospira spp., Listeria spp., Salmonella spp., Chlamydophila spp., Mycoplasma spp. and a variety of viruses and mycotic agents. Coxiella burnetii also cause abortion in ruminants, which is less considered in Iran. To detect the role of Coxiella burnetii in small ruminant's abortion in Hamedan province, 31 samples from liver and kidney and also abomasum contents of aborted fetuses of sheep and goats are taken and test with PCR method. The results of whole samples were negative. Since comprehensive epidemiological study has not been conducted in Hamadan and PCR test was done only on fetus samples referring to the central veterinary laboratories and also the limited number of samples so negative results in this study cannot be the reason for the lack of C. burnetii in the Hamedan province.

Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Recombinant antibodies are new generation of monoclonal antibodies, which are isolated via phage display technology from immune or non-immune phage libraries against target antigens. These antibodies are used for diagnosis of many different antigens and therapeutics proposes. The object of the present study was to isolate recombinant monoclonal antibodies against this important parasite. The purified Toxoplasma gondii surface antigen, P30, was coated to immunotubes and used as a target for selection of antibodies from the Tomlinson I and J phage display libraries of single-chain fragment variable (scFv) antibodies. Clones that were able to recognize antigen were isolated in three rounds of binding, elution and amplification. The specificity of scFv antibodies chosen from the resulting panel, were confirmed using enzyme-linked immunosorbent assay (ELISA), dot blotting and western blotting methods. Recombinant antibodies capable of recognizing P30 antigen were isolated with high affinity; and their specificity was approved. Isolated soluble single chain antibodies are good candidates to apply as monoclonal recombinant antibodies in diagnostic kits for detection of Toxoplasma gondii in contaminated samples.

Cryptosporidiosis is a zoonotic coccidian disease that is highly prevalent in the world. This is a parasitic infection and causing acute digestive disorders (diarrhea) in man and animals. The pigeons probably may have a role in the transmission of Cryptosporidium infection for other birds and human. This study was carried out to determine the prevalence of Cryptosporidium spp infection in pigeons in Mashhad from October 2008 to September 2009. Faecal samples from 200 pigeons were collected. Detection of oocysts was performed using the formalin- ether sedimentation method and the modified Ziehl-Neelsen staining technique. Cryptosporidium oocysts were identified in 2.5 % of the samples. The highest rate of infection was in winter although this difference was not statistically significant. Cryptosporidium oocysts were identified in three female and tow male pigeons. No significant difference was observed between prevalence in male and female pigeons. Diarrhea was recorded in three of the positive pigeons. Prevalence was not significantly higher in diarrheic pigeons versus non-diarrheic pigeons. The results showed that local pigeons probably may have a role in the transmission of Cryptosporidium infection for other birds and human.