فهرست مطالب سهراب احمدی وند

Infectious pancreatic necrosis is caused by IPNV virus that represented by various strains with different virulence linked to some motifs of the VP2 capsid. In this study (2018-2019), we screened some suspected Iranian trout farms for IPNV, and the genotype and virulence motifs of the positive samples were characterized. The causative agents of two outbreaks in trout farms of Kermanshah and Hamedan provinces in Iran were diagnosed as IPNV by RT-PCR assay. Phylogenetic analysis of VP2 gene revealed the detected IPNV isolates belonged to the Genotype 5 (Sp serotype) with close identity (> 99%) with European, Turkish, as well as previously reported Iranian isolates. Characterization of VP2 from both outbreaks has also shown the detected isolates have proline and threonine at amino acid residues 217 and 221, respectively, which are associated with low pathogenicity. In conclusion, the IPNV isolates from some Iranian trout farms (Kermanshah and Hamedan provinces) are of Sp serotype (Genotype 5) that despite P217T221 motif show moderate virulence, and may have originated from Europe via eyed eggs.

Bacterial infections of the genital system are common causes of infertility. One of the most important causes of male infertility is seminal and genital tract infections. In the meantime, a wide range of bacteria, in varying degrees, contribute to infertility in men. Helicobacter pylori may be involve in infertility. Because antibodies against this bacteria have been significantly detected in the genital fluids of infertile patients. The aim of this study was to determine the presence of Helicobacter pylori in the semen of the infertile men with rapid molecular PCR method and determine its percentage.

100 samples of semen collected from Saram hospital and DNA Extracted from these specimens using Boiling/DNG_PLUS method.  Optimized PCR test was performed on samples. PCR test evaluated from the respect sensitivity and specificity.

In this study, amplicon with the size of 294 bp, observed with agarose electrophoresis.  In the specificity test, primers created only a band for H.pylori DNA. Of the 100 samples tested, only 10 samples (10%) were positive for DNA of H.pylori.

According to the results of this study, H.pylori may be one of the bacterial agents that can be considered for male infertility, of course requires further studies. While PCR is suitable, quick and sensitive molecular technique for detection of H.pylori in infertile men.

The zoonotic bacteria, Streptococcus iniae is the causative agent of streptococcosis associated with serious economic losses in the word aquaculture industry. The antimicrobial activities of Thymus vulgaris, Carum carvi, and Menthalong flora essential oils were determined against two pathogenic isolates of S. iniae using broth microdilution, and were compared with erythromycin and enrofloxacin antibiotics by disk diffusion assay. Also, bacterial growth was followed by OD at 600 nm. The minimum inhibitory concentration (MIC) of the T. vulgaris, C. carvi, and M. flora essential oils were 0.8, 0.8, and 0.8-1.6 μl/ml, and the minimum bactericidal concentration (MBC) values were found as 0.8, 1.6, and 1.6 μl/ml, respectively. Moreover, the growth of isolates was reduced by increasing essential oils concentration. Also, disk diffusion assay revealed that the antimicrobial activity of T. vulgaris essential oil is significantly higher than the other plants, as well as erythromycin and enrofloxacin antibiotics. These findings support the use of T. vulgaris for the control of streptococcosis in trout farms.