فهرست مطالب سید سینا باقری

Staphylococcus aureus is a highly versatile pathogen of a large number of domestic ani- mals, including avian species. There is limited information about S. aureus isolated from companion and wild birds in Iran.

The aim of this study was to determine drug resistance and random-amplified polymorphic DNA-PCR (RAPD-PCR) pattern of S. aureus isolated from birds referred to the pet birds’ clinic of University of Tehran.

During the study period, 53 isolates of S. aureus were recovered from companion birds of var- ious species using standard bacteriologic procedures and the respective drug resistance patterns were deter- mined for a panel of 30 antimicrobial agents by agar disk-diffusion method. RAPD-PCR was performed with two different 10-bp oligonucleotide primers in a duplex-PCR procedure.

The findings of this study demonstrated that S. aureus resistance to oxacillin, clindamycin and methicillin were 58, 53 and 53%, respectively. The multi-drug resistance (MDR) was found among all isolates. The MDR pattern was variable and ranged from 0 to 17 drugs. In total, all 53 isolates generated 43 different resistance patterns. In RAPD-PCR, five different patterns of A, B, C, D and E were found. Among 53 isolates, 20, 62, 3, 9 and 3% belonged to RAPD patterns of A, B, C, D and E, respectively.

This study showed the widespread antimicrobial resistance among S. aureus isolated from pet birds; in particular, the presence of MRSA isolates. The value of RAPD-PCR for epidemiologic monitoring of S. aureus in pet birds also was noticed

Gammacoronaviruses, which are single-stranded, positive-sense RNA viruses, are responsible for a wide variety of existing and emerging diseases in birds. The Gammacoronaviruses primarily infect avian hosts.

This study aimed to investigate the genetic diversity of Gammacoronaviruses in quail population in Iran.

In the period from 2016 to 2018, samples from 47 quail flocks with or without enteric signs, were collected from four provinces in Iran.

Gammacoronavirus was detected in samples of 4 flocks by using RT-PCR and characterized by N gene sequencing. The isolates formed a distinct group from other Gamma- coronaviruses groups.

The finding suggests the existence of a novel Gammacoronavirus circulating in quail farms. The phylogenetic relationship of the isolates concerning different sequences and geographical regions displayed complexity and diversity. The present study is the first detection of Gammacoronavirus in quail farms in Iran. Further studies are required and should include the isolation and experimental studies of Gammacoronaviruses in Iran.